Global metabolomic and lipidomic analysis reveals the potential mechanisms of hemolysis effect of Ophiopogonin D and Ophiopogonin D' in vivo

Abstract Background OPD and OPD' are the two main active components of Ophiopogon japonicas in Shenmai injection (SMI). Being isomers each other, they supposed to have similar pharmacological activities, but actual situation is complicated. The difference hemolytic behavior between vivo vitro was discovered reported by our group for first time. In vitro, only showed hemolysis reaction, while vivo, both caused hemolysis. primary cause has been confirmed be related physical chemical properties OPD'. although there a possible explanation this phenomenon, one that bio-transformed into or its analogues other were metabolized more activated forms However, mechanism still unclear, especially existing literature difficult explain why shows inconsistent vitro. Therefore, study great practical significance response increase adverse events SMI. Methods Aiming at manuscript adopted untargeted metabolomics lipidomics technology preliminarily explore changes plasma metabolites lipids OPD- OPD'-treated rats. Metabolomics analyses performed on ultra-high performance liquid chromatography (UPLC) system tandem with different mass spectrometers (MS) columns respectively. Multivariate statistical approaches such as principal component analysis (PCA) orthogonal partial least square-discriminant (OPLS-DA) applied screen differential lipids. Results Both groups experienced hemolysis, Changes endogenous lipids, enrichment metabolic pathways, correlation all indicated causes closely interference phospholipid metabolism. Conclusions This provided comprehensive description rats, it would add knowledge base field, which also scientific guidance subsequent research. underlying require further